Journal
EPIGENOMICS
Volume 5, Issue 4, Pages 439-452Publisher
FUTURE MEDICINE LTD
DOI: 10.2217/EPI.13.37
Keywords
approach; chromatin immunoprecipitation; DNA methylation; epigenetic; genomic; histone modification; mass spectrometry; proteomic
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Funding
- National Science Foundation Early Faculty CAREER award grant
- NIH New Innovator award grant from the Office of the Director [DP2OD007447]
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [1262672] Funding Source: National Science Foundation
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Epigenetics is the study of changes in gene expression or cellular phenotype that do not change the DNA sequence. In this review, current methods, both genomic and proteomic, associated with epigenetics research are discussed. Among them, chromatin immunoprecipitation (ChIP) followed by sequencing and other ChIP-based techniques are powerful techniques for genome-wide profiling of DNA-binding proteins, histone post-translational modifications or nucleosome positions. However, mass spectrometry-based proteomics is increasingly being used in functional biological studies and has proved to be an indispensable tool to characterize histone modifications, as well as DNA-protein and protein-protein interactions. With the development of genomic and proteomic approaches, combination of ChIP and mass spectrometry has the potential to expand our knowledge of epigenetics research to a higher level.
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