Journal
EPIGENOMICS
Volume 2, Issue 2, Pages 187-197Publisher
FUTURE MEDICINE LTD
DOI: 10.2217/EPI.09.48
Keywords
chromatin; histone modifications; next-generation sequencing nucleosome positioning
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Funding
- NATIONAL HUMAN GENOME RESEARCH INSTITUTE [U01HG004258] Funding Source: NIH RePORTER
- NHGRI NIH HHS [U01 HG004258-02, U01 HG004258] Funding Source: Medline
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The recent development of next-generation sequencing technology has enabled significant progress in chromatin structure analysis. Here, we review the experimental and bioinformatic approaches to studying nucleosome positioning and histone modification profiles on a genome-wide scale using this technology. These studies advanced our knowledge of the nucleosome positioning patterns of both epigenetically modified and bulk nucleosomes, and elucidated the role of such patterns in the regulation of gene expression. The identification and analysis of large sets of nucleosome-bound DNA sequences allowed a better understanding of the rules that govern nucleosome positioning in organisms of varying complexity. We also discuss the existing challenges and prospects of using next-generation sequencing for nucleosome positioning analysis and outline the importance of such studies for the entire chromatin structure field.
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