Journal
EXPERIMENTAL AND THERAPEUTIC MEDICINE
Volume 9, Issue 2, Pages 553-558Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/etm.2014.2099
Keywords
nitric oxide; rotenone; Parkinson's disease
Categories
Funding
- Natural Science Foundation of Zhejiang Province [Y2100035, Y2111046]
- Key Medical Discipline Construction Project of Zhejiang Province [GJSX-010-004]
- Science and Technology Development Project of Zhejiang Province [2010C33116]
- Science and Technology Development Project of Shaoxing, Zhejiang Province [2010A23021]
- Zhejiang Provincial Administration of Traditional Chinese Medicine [2011ZA108, 2012ZB162]
- Department of Education of Zhejiang Province [Y201018615]
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Systemic rotenone models of Parkinson's disease (PD) are highly reproducible and may provide evidence on the pathogenesis of PD. In the present study, male Sprague-Dawley rats (1-year-old) were subcutaneously administered with rotenone (1.5 mg/kg/day) for six days and observed for the following three weeks. Compared with the control rats, a significant decrease was observed in the body weight and a marked increase was observed in the areas under the behavioral scoring curves in the rotenone-treated rats. Immunohistochemical staining revealed that the abundance of nigral tyrosine hydroxylase (TH)-positive neurons was markedly reduced following rotenone treatment. ELISA and neurochemical assays demonstrated a significant increase in the levels of nitric oxide (NO) and NO synthase, whereas a marked decrease was observed in the thiol levels in the brains of the rotenone-treated rats. Thus, subacute rotenone treatment was found to induce behavioral deficits and the loss of nigral TH-positive neurons which may be associated with the excessive levels of NO in the rat brains.
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