Journal
ALTEX-ALTERNATIVES TO ANIMAL EXPERIMENTATION
Volume 31, Issue 1, Pages 43-52Publisher
SPEKTRUM AKADEMISCHER VERLAG-SPRINGER-VERLAG GMBH
DOI: 10.14573/altex.1309181
Keywords
IgY antibody; breast cancer; CA 15-3 antigen; ELISA
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Funding
- Polish research funding agency
- National Center for Research and Development [LIDER/08/90/1-1/09/NCBiR/2010]
- European Union as part of the European Social Fund
- Wroclaw University of Technology (Statute Funds) [S30134/Z0313]
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Early detection of cancer development is crucial for successful therapy and for monitoring patient outcome. Various immunodiagnostic methods are able to detect pathological changes in the human body ahead of symptomatic manifestation of the disease. Most immunological examinations are based on the detection of specific tumor markers in body fluids. Of the various cancer-specific proteins used for breast cancer diagnostics, one of the most commonly applied is the cancer antigen 15-3 (CA 15-3). An elevation in its serum level (>25-40 U/ml) usually correlates with tumor malignancy. The CA 15-3 antigen is also used for monitoring patients after surgical treatment and for measuring therapeutic efficacy. Herein, we present the generation of polyclonal IgY antibodies isolated from egg yolks of immunized hens and their application for CA 15-3 detection. The developed sandwich ELISA assay showed a detection limit of 0.028 U/ml, thus demonstrating its potential for clinical applications.
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