Journal
CELL DEATH & DISEASE
Volume 4, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/cddis.2013.184
Keywords
miR-29; myoblast; proliferation; differentiation; Akt3
Categories
Funding
- National Key Basic Research Program of China [2012CB124702]
- National Outstanding Youth Foundation of the National Natural Science Foundation of China [31025026]
- Youth Foundation of the National Natural Science Foundation of China [30901020]
- Biotechnology and Biological Sciences Research Council (UK) China Partnering Award
- BBSRC [BB/I024704/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/I024704/1] Funding Source: researchfish
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MicroRNAs (miRNAs) are a type of endogenous noncoding small RNAs involved in the regulation of multiple biological processes. Recently, miR-29 was found to participate in myogenesis. However, the underlying mechanisms by which miR-29 promotes myogenesis have not been identified. We found here that miR-29 was significantly upregulated with age in postnatal mouse skeletal muscle and during muscle differentiation. Overexpression of miR-29 inhibited mouse C2C12 myoblast proliferation and promoted myotube formation. miR-29 specifically targeted Akt3, a member of the serine/threonine protein kinase family responsive to growth factor cell signaling, to result in its post-transcriptional downregulation. Furthermore, knockdown of Akt3 by siRNA significantly inhibited the proliferation of C2C12 cells, and conversely, overexpression of Akt3 suppressed their differentiation. Collectively and given the inverse endogenous expression pattern of rising miR-29 levels and decreasing Akt3 protein levels with age in mouse skeletal muscle, we propose a novel mechanism in which miR-29 modulates growth and promotes differentiation of skeletal muscle through the post-transcriptional downregulation of Akt3.
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