Journal
MINE WATER AND THE ENVIRONMENT
Volume 32, Issue 4, Pages 258-265Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s10230-013-0250-8
Keywords
Microbial iron oxidation; Live/dead-stain; Microbial community analysis; Ferrovum; Saxony; Germany
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Funding
- Federal Ministry of Education and Research (BMBF) within the Geotechnologien RD program [03G0714B]
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Treatment of acidic iron-and sulfate-rich mine waters in a pilot plant at the opencast lignite mining pit Nochten (Lusatia, Germany) involves microbial iron oxidation and subsequent precipitation of the iron-oxyhydroxysulfate schwertmannite. To determine if recirculation of schwertmannite can stabilize and optimize the oxidation process, cell number, viability, and diversity of the bacterial community in schwertmannite precipitated on carrier material and stored schwertmannitewere analyzed. In schwertmannite on carrier material, the total cell number decreased slightly with increasing mineral depth, whereas the percentage of viable cells decreased significantly. The microbial community, investigated by fluorescence-in-situ-hybridization (FISH) and terminal restriction fragment length polymorphism (T-RFLP), revealed the presence of the iron-oxidizing bacterium Ferrovum sp. and relatives of Gallionella, independent of mineral depth. Analysis of the viability of microorganism in stored schwertmannite indicated an almost constant percentage of viable cells over 42 days.
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