Journal
JOURNAL OF FUNCTIONAL FOODS
Volume 8, Issue -, Pages 331-339Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jff.2014.03.026
Keywords
Fluorescence spectroscopy; Interaction; Lysozyme; Naringin; Naringin palmitate
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Funding
- State Key Program of the National Natural Science of China [31130042, 31371833]
- National Key Technology RD Program [2012BAD37B01]
- Program for New Century Excellent Talents in University [NCET-10-0395]
- Open Project Program of Process of Starch and Vegetable Protein Engineering Research Center of Ministry of Education
- Fundamental Research Funds for the Central Universities, SCUT [2014ZB0006]
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Naringin palmitate is an acylated product of naringin with palmitic acid, which is an effective substance for enhancing naringin lipophilicity. Lysozyme has strong ability to carry drugs and functional compounds in mammals. Herein, the ability of lysozyme to carry naringin palmitate was investigated by fluorescence and UV spectroscopy, and the differences in affinities of naringin and naringin palmitate for lysozyme were compared. Naringin palmitate was found to quench the fluorescence of lysozyme via static quenching. The binding constant and number of binding sites of naringin palmitate-lysozyme were more sensitive to high temperature than naringin-lysozyme. van der Waals forces and hydrogen bond formation played a major role in stabilizing naringin palmitate-lysozyme. No significant change of the structure and microenvironment of lyzozyme were obtained by addition of naringin palmitate. Thus, transport of naringin palmitate by lysozyme was verified. These findings are of importance and are attractive for the practical applications of naringin palmitate. (C) 2014 Elsevier Ltd. All rights reserved.
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