Journal
JOURNAL OF FUNCTIONAL FOODS
Volume 7, Issue -, Pages 719-726Publisher
ELSEVIER
DOI: 10.1016/j.jff.2013.12.013
Keywords
Bioactive peptide; Caco-2; HT-29; Antioxidant enzymes; Nrf2
Categories
Funding
- Nature Science Foundation of China [31101230]
- Project of Jiangsu Province [BE2011704]
- Fundamental Research Project Funds of the Chinese Ministry of Education [2060204]
Ask authors/readers for more resources
The effects of an antioxidative pentapeptide from chickpea protein hydrolysates on oxidative stress in Caco-2 and HT-29 cell lines were investigated. The bioactive pentapeptide had an amino acid sequence of Asn-Arg-Try-His-Glu (NRYHE). Caco-2 and HT-29 cells were pretreated with peptide (0.05, 0.1, 0.25 and 0.5 mg/mL) for 2 h and stimulated with 1 mM H2O2 for 6 h. The activity of three important antioxidative enzymes, catalase, glutathione reductase and glutathione peroxidase, increased in a concentration-dependent manner. It was observed that peptide treatment elevated the expression of Nrf2 mRNA and several relative genes NQO1, HO-1, gamma-GCS regulated by Nrf2 compared to the positive control. The trends in HT-29 and Caco-2 cells were similar. (C) 2013 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available