Journal
BIOANALYSIS
Volume 10, Issue 17, Pages 1427-1438Publisher
FUTURE SCI LTD
DOI: 10.4155/bio-2018-0082
Keywords
ELISA; hybrid assay; immunocapture; LC-MS; magnetic beads; monkey; plasma; streptavidin plate
Funding
- Boehringer Ingelheim Pharmaceuticals, Inc.
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Background: Magnetic bead immunocapture-LC-MS has been widely used for bioanalysis of biotherapeutic proteins. However, magnetic beads are difficult to be fully automated and more costly than ELISA plates. Aim: Develop an ELISA-LC-MS hybrid assay as an alternate platform. Results: Among seven ELISA plates tested, Pierce streptavidin plates, which did not require time-consuming capture antibody precoating steps, provided the best sensitivity and assay dynamic range (5-2500 ng/ml or 10-5000 ng/ml), similar to magnetic bead immunocapture-LC-MS assay and better than an ELISA (50-500 ng/ml). The entire procedures could be fully automated using a liquid handling system. Conclusion: This study demonstrates that ELISA-LC-MS hybrid approach using streptavidin plates represents a promising platform for bioanalysis of biotherapeutics.
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