Recent advances in liquid microextraction techniques coupled with MS for determination of small-molecule drugs in biological samples

Sample preparation is an important and necessary step in a measurement process for isolation and concentration of desired components from complex matrices. It is the most time-consuming and error-prone step in analytical methodology, greatly affecting quality and quantity of analytical data. During the past 15 years, solvent microextraction techniques have been introduced as alternatives to conventional sample preparation methods, such as liquid-liquid extraction and solid-phase extraction. These novel methodologies, which have proved to be extremely simple, low-cost and virtually solvent-free sample-preparation techniques provide a high degree of selectivity, sample cleanup and enrichment. The aim of the present review is to explore recent analytical applications of solvent microextraction techniques for quantification of drugs in biological samples, with particular focus on the methods involving MS as a detection system.