Journal
ENVIRONMENTAL MICROBIOLOGY REPORTS
Volume 7, Issue 2, Pages 204-210Publisher
WILEY-BLACKWELL
DOI: 10.1111/1758-2229.12227
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Funding
- Genomic Science Program (GSP), Office of Biological and Environmental Research (OBER), US Department of Energy (DOE)
- Russian Science Foundation [14-14-00289]
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Bacteria from the Chloroflexi phylum are dominant members of phototrophic microbial mat communities in terrestrial thermal environments. Vitamins of B group are key intermediates (precursors) in the biosynthesis of indispensable enzyme cofactors driving numerous metabolic processes in all forms of life. A genomics-based reconstruction and comparative analysis of respective biosynthetic and salvage pathways and riboswitch regulons in over 20 representative Chloroflexi genomes revealed a widespread auxotrophy for some of the vitamins. The most prominent predicted phenotypic signature, auxotrophy for vitamins B-1 and B-7 was experimentally confirmed for the best studied model organism Chloroflexus aurantiacus. These observations along with identified candidate genes for the respective uptake transporters pointed to B vitamin cross-feeding as an important aspect of syntrophic metabolism in microbial communities. Inferred specificities of homologous substrate-binding components of ABC transporters for vitamins B-1 (ThiY) and B-2 (RibY) were verified by thermofluorescent shift approach. A functional activity of the thiamine-specific transporter ThiXYZ from C.aurantiacus was experimentally verified by genetic complementation in E.coli. Expanding the integrative approach, which was applied here for a comprehensive analysis of B-vitamin metabolism in Chloroflexi would allow reconstruction of metabolic interdependencies in microbial communities.
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