Journal
ENVIRONMENTAL MICROBIOLOGY REPORTS
Volume 4, Issue 1, Pages 42-49Publisher
WILEY-BLACKWELL
DOI: 10.1111/j.1758-2229.2011.00277.x
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Funding
- Japan Science Society
- KAKENHI [22740351]
- MEXT
- 21st Century COE, Tokyo Institute of Technology
- Ministry of Education Culture, Sports, Technology and Science, Japan
- Grants-in-Aid for Scientific Research [21244077, 20109005, 22540499, 22740351, 20224012] Funding Source: KAKEN
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Domain-level identification of microbial cells or cell-like structures is crucial for investigating natural microbial communities and their ecological significance. By using micro-Fourier transform infrared (micro-FTIR) spectroscopy, we established a technical basis for the domain-level diagnosis and quantification of prokaryotic cell abundance in natural microbial communities. Various prokaryotic cultures (12 species of bacteria and 10 of archaea) were examined using micro-FTIR spectroscopic analysis. The aliphatic CH3/CH2 absorbance ratios (R-3/2) showed domain-specific signatures, possibly reflecting distinctive cellular lipid compositions. The signatures were preserved even after chemical cell fixation (formaldehyde) and nucleic acid staining (DAPI) processes - techniques that are essential in studying microbial ecology. The micro-FTIR technique was successfully applied for quantification of the bacteria/archaea abundance ratio in an active microbial mat community in a subsurface hot aquifer stream. We conclude that the micro-FTIR R3/2 measurement is both fast and effective for domain-level diagnosis and quantification of first-order prokaryotic community structures.
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