Journal
ENVIRONMENTAL MICROBIOLOGY REPORTS
Volume 1, Issue 5, Pages 442-449Publisher
WILEY
DOI: 10.1111/j.1758-2229.2009.00069.x
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Funding
- European Community [ERBCHGE-CT95-0066]
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P>An obligate methanotrophic bacterium, strain MTS, was isolated from a methane-fed microaerobic denitrifying bioreactor. 16S rRNA and DNA-DNA hybridization analysis revealed that this organism was most closely related to Methylocystis parvus, a Type II methanotroph, belonging to the alpha-subclass of the Proteobacteria. The metabolism of the bacterium under microaerobic and anaerobic conditions was studied by 13C-NMR. 13C-labelled poly-beta-hydroxybutyrate (PHB) formation occurred in cell suspensions incubated with 13C-labelled methane at low (5-10%) oxygen concentration. Under these conditions low levels of succinate, acetate and 2,3-butanediol were formed and excreted into the culture medium. Intracellular PHB degradation was observed in intact cells under anaerobic conditions in the absence of an exogenous carbon source during a long-term incubation of 90 days. Multiple 13C-labelled beta-hydroxybutyrate, butyrate, acetate, acetone, isopropanol, 2,3-butanediol and succinate were identified as products in in vivo13C-NMR spectra and in the spectra of culture medium during the dynamic PHB degradation. The isolated obligate methanotroph clearly shows a fermentative metabolism of PHB under anaerobic conditions. The excreted products may serve as substrates for denitrifying bacteria.
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