Journal
JOURNAL OF MATERIALS CHEMISTRY B
Volume 3, Issue 15, Pages 3091-3097Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5tb00207a
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Funding
- 973 Projects [2012CB933803, 2014CB643605]
- National Science Fund for Distinguished Young Scholars [50925310]
- National Science Foundation of China [21374060, 51173103]
- Excellent Academic Leaders of Shanghai [11XD1403000]
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Two-photon fluorescence imaging has attracted increasing interest in the biological and medical fields because of its low cell damage, high resolution, large imaging depth, and easy dynamic observation. A high-performance two-photon probe with long-term imaging capability was proposed for this imaging technology. In this work, a new two-photon probe compound was synthesized from tetraphenylethylene fluorogen with aggregation-induced emission. A phenyl-[phenyl-(1,2,5-thialdiazol)] amine group was induced to red-shift the absorption and emission wavelengths of the compound. After self-assembly, fluorescent dots with aggregation-induced emission cores and hydrophobic shells terminated by -COOH were formed. Cell experiments proved that the 4-(7-(phenyl(4-(1,2,2-triphenylvinyl) phenyl) amino) benzo[c][1,2,5] thiadiazol4-yl) benzoic acid (TPECOOH) dots with red emission showed good biocompatibility and excellent two-photon imaging ability. TPECOOH dots were used successfully in direct long-term cell imaging with high efficiency. Even after twelve days, fluorescence imaging could still be observed in live HeLa cells.
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