Journal
JOURNAL OF MATERIALS CHEMISTRY B
Volume 3, Issue 7, Pages 1335-1341Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4tb01620c
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Funding
- National Basic Research Program of China [2010CB732400]
- National Natural Science Foundation of China [21375060, 21121091, 21135002]
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In this work, a kind of biomimetic catalyst was designed as a signal probe by the assembly of porphyrin on porous carbon (PC) derived from metal-organic frameworks for electrochemical biosensing. First, PC was prepared by direct carbonization of a zeolite-type metal-organic framework as both a precursor and a template, and characterized by transmission electron microscopy, nitrogen sorption isotherm, X-ray photoelectron spectroscopy and X-ray diffraction. Then the PC was non-covalently functionalized with iron(III) meso-5,10,15,20-tetrakis(4-carboxyphenyl) porphyrin chloride (FeTCPP) via non-covalent interactions. The resulting nanocomposite of PC/FeTCPP possesses an excellent electrocatalytic activity toward oxygen reduction. Coupling with the enzymatic catalysis of glucose oxidase, a glucose biosensor was constructed on the basis of O-2 consumption. The biomimetic sensor enables a reliable and sensitive determination of glucose with a linear range of 0.5 to 18 mM and a detection limit of 0.08 mM at a signal-to-noise ratio of 3. Moreover, the biosensor exhibits the analytical reproducibility and stability with a lower relative standard deviation of 4.2%. In an application to detect glucose in human serum samples, this glucose biosensor had good detection accuracy with analytical recoveries from 97.3% to 107.7%. Therefore, the porphyrin functionalized PC provides a promising biomimetic platform for constructing the biosensors, and has potential application in bioanalysis and clinical diagnosis.
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