Journal
Journal of Materials Chemistry B
Volume 3, Issue 17, Pages 3465-3475Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4tb02070g
Keywords
-
Categories
Funding
- Mid-career Research program through the National Research Foundation of Korea (NRF) - Ministry of Education, Science and Technology (MEST) [2011-0028922]
- FCT, Portugal [SFRH/BPD/86507/2012]
Ask authors/readers for more resources
Gene therapy is the treatment of human disorders by the introduction of genetic material to specific target cells of a patient. Chitosan and its derivatives show excellent biological properties including biocompatibility, biodegradability and nonallergenicity. Primary amines of chitosan are responsible for its cationic nature and hence binding and protection of DNA for intracellular delivery. But the transfection efficiency of chitosan based gene transporters is severely hampered by its poor physical properties such as low water solubility and high viscosity. In this study, primary amines of low molecular weight (LMW) chitosan were coupled with 2-acrylamido-2-methylpropane sulphonic acid (AMP) making it water soluble for its application in gene delivery. AMP modified chitosan (CSAMP) showed an enhanced interaction with DNA and a higher buffering capacity due to AMP amines leading to a higher transfection efficiency in cancer cells (A549, HeLa and HepG(2)) compared to native chitosan and Lipofectamines (R). In vivo studies in Balb/c through intravenous injection demonstrated a higher luciferase expression compared to LMW chitosan.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available