Journal
CHEMICAL SCIENCE
Volume 5, Issue 9, Pages 3368-3373Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4sc01417k
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Funding
- China Scholarship Council (CSC)
- University of Bath Full Fees Scholarship
- ECUST
- University of Birmingham
- National Research Foundation of Korea (NRF) grants - Korean government (MSIP) [2012R1A3A2048814]
- National Research Foundation of Korea [2012R1A3A2048814] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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A water-soluble boronate-based fluorescent probe was evaluated for the detection of peroxynitrite (ONOO-) in the presence of a monosaccharide. The enhanced fluorescence of the probe when bound with o-fructose was switched off in the presence of peroxynitrite. In contrast, other reactive oxygen/nitrogen species (ROS/RNS) led to only slight fluorescence decreases due to protection by an internal N-B interaction. The interaction of the probe with o-fructose not only strengthens the fluorescence signal, but also protects the boronic acid from oxidation by other ROS/RNS. Therefore, under conditions generating various ROS/RNS, the boronate-based saccharide complex preferentially reacts with peroxynitrite. The probe was used in cell imaging experiments for the detection of endogenous and exogenous peroxynitrite. The sensor displays good on-off responses towards peroxynitrite, both in RAW 264.7 cells and HeLa cells.
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