Journal
CHEMICAL SCIENCE
Volume 5, Issue 8, Pages 3183-3188Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4sc00838c
Keywords
-
Categories
Funding
- Natural Science Foundation of China [21172137, 21302114]
- Specialized Research Fund for the Doctoral Program of Higher Education (SRFDP) [20101401110010]
- Program for New Century Excellent Talents in University [NCET-11-1034]
Ask authors/readers for more resources
A 4-methoxythiophenol-substituted pyronin dye 1 was exploited as reaction-type fluorescent probe for biothiols Cys/Hcy and GSH. The probe itself is nonfluorescent due to the photoinduced electron transfer (PET) process. The Cys (or Hcy)-induced substitution-rearrangement cascade reaction and GSH-induced substitution reaction with the probe lead to the corresponding aminopyronin and thiopyronin dyes with distinct photophysical properties, enabling Cys/Hcy and GSH to be detected from visible and near-infrared (NIR) emission channels, respectively, in pure PB buffer with relatively fast kinetics and obvious fluorescence turn-on response. Assisted by laser scanning confocal microscope, we also demonstrated that probe 1 could simultaneously sense Cys/Hcy and GSH in B16 cells in multicolor imaging.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available