Journal
JOURNAL OF MATERIALS CHEMISTRY B
Volume 3, Issue 48, Pages 9260-9268Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5tb01602a
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Funding
- Beijing Municipal Science & Technology Commission [Z141100002114049]
- TJSHG [201310025008]
- IHLB [KZ20121-0025021]
- National Natural Science Foundation [81172930, 81273379, 81373264, 81373265, 81270046, 81502688]
- Joint Research and Development of protective agents for low dose radiation injury [2014DFR30930]
- Importation and Development of High-Caliber Talents Project of Beijing Municipal Institutions
- Natural Science Foundation of Capital Medical University [2015ZR14]
- China's 55 of postdoctoral scientific research funds
- [PXM2013-014226 000002]
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A nonviral tumor targeting vector for siRNA transfer is of importance. Here, a novel delivery system consisting of a covalent conjugate of NDCO-RGDS and VEGF-siRNA, NDCO-RGDS/VEGF-siRNA, was presented. In vitro, NDCO-RGDS/VEGF-siRNA released and transferred VEGF-siRNA in a long-acting manner. Compared to the control, NDCO-RGDS/VEGF-siRNA decreased the expression of VEGF mRNA and protein in HeLa cells by 88.41 +/- 3.49% and 83.94 +/- 2.00%, respectively. In vivo, NDCO-RGDS/VEGF-siRNA exhibited gene silencing and slowed tumor growth. FT-MS spectrum analysis revealed that NDCO-RGDS/VEGF-siRNA mainly distributed in tumor tissue of the treated S180 mice. Therefore NDCO-RGDS could be considered a promising nonviral tumor-targeting vector for siRNA transfer in tumor therapy.
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