Journal
CHEMICAL SCIENCE
Volume 3, Issue 6, Pages 1780-1784Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2sc01119k
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Funding
- National Key Basic Research Foundation of China [2010CB923303, 2012CB917300]
- National Natural Science Foundation of China [20902045, 91013009, 21001010]
- Jiangsu Planned Projects for Postdoctoral Research Funds [1001056C]
- Fok Ying-Tong Education Foundation, China [132011]
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A gold-specific sensory protein GolS from Samonella gol regulon was incorporated into E. coli, which in conjunction with an engineered downstream red fluorescence protein allowed the highly sensitive and selective whole-cell detection of gold(III) ions by naked eyes. The putative gold-chaperone, GolB, in the gol regulon was next verified to be specific to gold(I) ions over other metal ions including copper(I). The subsequent display of GolB on E. coli cell surface permitted selective enrichment of gold ions from media containing various thiophilic metal ions. The cell surface-enriched gold(I) was further shown to be easily recovered and the gold-deprived bacteria were capable for re-usage. E. coli bacteria harboring these gold-specific elements from the gol regulon could be a valuable tool for visual detection and facile recycling of gold ions from environmental resources.
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