Journal
CHEMICAL SCIENCE
Volume 3, Issue 3, Pages 711-722Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2sc00630h
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Funding
- Glasgow/Strathclyde Synergy Fund, through a Scottish Enterprise Proof of Concept Award
- Strathclyde Research Development Award
- Strathclyde Research Excellence Fund
- Strathclyde Centre for Physical Organic Chemistry
- EPSRC [EP/I037229/1] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [EP/I037229/1] Funding Source: researchfish
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DNA-sequence and structure dependence on the formation of minor groove complexes at 5'-XCYRGZ-3', where Y = T and R = A, by the short lexitropsin thiazotropsin A are explored based on NMR spectroscopy, isothermal titration calorimetry (ITC), circular dichroism (CD) and qualitative molecular modelling. The structure and solution behaviour of the complexes are similar whether X = A, T, C or G and Z = T, A, I (inosine) or C, 5'-CCTAGI-3' being thermodynamically the most favoured (Delta G = -11.1 +/- 0.1 kcal mol(-1)). Binding site selectivity observed by NMR for 5'-ACTAGT-3' in the presence of 5'-TCTAGA-3' when both accessible sequences are concatenated in a 15-mer DNA duplex construct is consistent with thermodynamic parameters (|Delta G|(ACTAGT) > |Delta G|(TCTAGA)) measured separately for the binding sites and with predictions from modelling studies. Steric bulk in the minor groove for Z = G causes unfavourable ligand-DNA interactions reflected in lower Gibbs free energy of binding (Delta G = -8.5 +/- 0.01 kcal mol(-1)). ITC and CD data establish that thiazotropsin A binds the ODNs with binding constants between 10(6) and 10(8) M-1 and reveal that binding is driven enthalpically through hydrogen bond formation and van der Waals interactions. The consequences of these findings are considered with respect to ligand self-association and the energetics responsible for driving DNA recognition by small molecules in the DNA minor groove.
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