Journal
CHEMICAL SCIENCE
Volume 1, Issue 4, Pages 447-452Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c0sc00229a
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Funding
- RCUK [EP/D048664/1]
- Engineering and Physical Sciences Research Council [EP/D048664/1] Funding Source: researchfish
- EPSRC [EP/D048664/1] Funding Source: UKRI
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Distinguishing specific cells is an essential technique in cell research and clinical diagnostics. We report a novel method to passively isolate and extract cells in a microfluidic device. We utilise a droplet-based microfluidic system to generate an aqueous two phase system in which aqueous droplets consist of two phases in the form of a double emulsion. Specifically, we generate PEG droplets that completely encapsulate DEX droplets within a microfluidic channel. Target cells can be introduced directly into the droplets and driven to partition to the more favourable phase, whilst still being contained within the aqueous droplet. Human T lymphoma cells, with diameters in the range of 10-15 mu m, are chosen as a model cell line to demonstrate the partitioning.
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