Journal
JOURNAL OF MATERIALS CHEMISTRY B
Volume 3, Issue 9, Pages 2006-2011Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4tb01695e
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Funding
- National Natural Science Foundation of China [21175057, 21375047, 21377046]
- Science and Technology Plan Project of Jinan [201307010]
- Special Foundation
- UJN [ts20130937]
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The excellent catalytic activity of a novel nanomaterial was introduced to ultrasensitive sandwich- type electrochemical immunosensor applications for detection of carcinoembryonic antigen (CEA). Amino-functionalized multi-walled carbon nanotube (MWCNT-NH2) supported PdPt nanocages were used as catalytic labels of secondary anti-CEA. PdPt nanocages with hollow interiors and porous walls could efficiently enhance electrocatalysis for reduction of H2O2. Meanwhile, with good dispersion, large specific surface area and amino groups, MWCNT-NH2 can immobilize a large amount of PdPt nanocages to improve the sensitivity and amplify the signal of the immunosensor. For the immobilization of primary antibodies, 3-aminopropyltriethoxysilane functionalized graphene sheets were used as transducing materials to modify glassy carbon electrodes and to enhance the electron transfer efficiently. Under the optimal conditions, the electrochemical immunosensor exhibited a wide linear range of 0.001-20 ng mL(-1) with a low detection limit of 0.2 pg mL(-1) for CEA. The proposed sensing strategy enriches electrochemical immunoassays and may have a promising application in bioassay analysis.
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