Journal
JOURNAL OF OPTICS
Volume 13, Issue 10, Pages -Publisher
IOP PUBLISHING LTD
DOI: 10.1088/2040-8978/13/10/105301
Keywords
optical trapping; water immersion objective; aberration; trap efficiency; infrared laser
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Focused infrared lasers are widely used for micromanipulation and visualization of biological specimens. An inherent practical problem is that off-the-shelf commercial microscope objectives are designed for use with visible and not infrared wavelengths. Less aberration is introduced by water immersion objectives than by oil immersion ones, however, even water immersion objectives induce significant aberration. We present a simple method to reduce the spherical aberration induced by water immersion objectives, namely by tuning the correction collar of the objective to a value that is similar to 10% lower than the physical thickness of the coverslip. This results in marked improvements in optical trapping strengths of up to 100% laterally and 600% axially from a standard microscope objective designed for use in the visible range. The results are generally valid for any water immersion objective with any numerical aperture.
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