Journal
JOURNAL OF OPTICS
Volume 13, Issue 4, Pages -Publisher
IOP Publishing Ltd
DOI: 10.1088/2040-8978/13/4/044021
Keywords
monocytes; Raman spectroscopy; laser tweezers; single-cell spectroscopy
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Funding
- National Science Foundation
- University of California, Davis [PHY 0120999]
- Keaton-Raphael Foundation for Childhood Cancer
- Clinical Translational Science Center from the National Center for Research Resources (NCRR), a component of the National Institutes of Health (NIH) [UL1 RR024146]
- NIH Roadmap for Medical Research
- LLNL Laboratory
- US Department of Energy by Lawrence Livermore National Laboratory [DE-AC52-07NA27344]
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We show that laser tweezers Raman spectroscopy of eukaryotic cells with a significantly larger diameter than the tight focus of a single-beam laser trap leads to optical trapping of the cell by its optically densest part, i.e. typically the cell's nucleus. Raman spectra of individual optically trapped monocytes are compared with location-specific Raman spectra of monocytes adhered to a substrate. When the cell's nucleus is stained with a fluorescent live cell stain, the Raman spectrum of the DNA-specific stain is observed only in the nucleus of individual monocytes. Optically trapped monocytes display the same behavior. We also show that the Raman spectra of individual monocytes exhibit the characteristic Raman signature of cells that have not yet fully differentiated and that individual primary monocytes can be distinguished from transformed monocytes based on their Raman spectra. This work provides further evidence that laser tweezers Raman spectroscopy of individual cells provides meaningful biochemical information in an entirely non-destructive fashion that permits discerning differences between cell types and cellular activity.
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