Journal
ASIAN PACIFIC JOURNAL OF TROPICAL MEDICINE
Volume 7, Issue -, Pages S93-S96Publisher
ELSEVIER SCI LTD
DOI: 10.1016/S1995-7645(14)60211-X
Keywords
Genetic resistance; Burkina Faso
Funding
- Union Monetaire Ouest Africaine (Programme PACER II)
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Objective: To study the involvement of variations in 4 genes associated with susceptibility and/or protection against HIV-1 in serodiseordant couples in Burkina Faso, namely, genes encoding HLA-B57, interferon regulatory factor 1 (IRF1), denthitie cell-specific ICAM3-grabbing nonintegrin (DC-SIGN) and CCR5 delta 32 (CCR5 Delta 32). Methods: Two DC-SIGN and two IRF1 single nucleotide polymorphisms (SNPs) as well as HLA-B57*01 and CCR5 Delta 32 alleles were genotyped in 51 serodiseordant couples in Burkina Faso. DC-SIGN, IRF1 and HLA-B57*01 genotyping was carried out by real time PCR using TaqMan assays (Applied Biosystems, USA and Sacace Biotechnologies, CCR5 Delta 32 deletion was investigated by PCR. Results: The two sNPs of DC-SIGN promoter showed a significant genotypic difference in serodiscordant couples. After multivariate analysis, only the association between DC-SIGN rs2287886 and HIV-1 remained significant (P<0.01). No association was found between IRF1 SNPs and HIV-1 infection. CCR5 Delta 32 wild type allele was found in 100% of serodiseordant couples. A high frequency of HLA-B57*01 allele was found in the HIV-positive (78%) compared with HIV-negative group (51%), however this difference was no longer significant after the correction of the sex confounding effect in the logistic regression model. Conclusions: Our study suggests a protective role of a variation of DC-SIGN promoter and genetic resistance to HIV-1 in serodiseordant couples in Burkina Faso.
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