Journal
ASIAN PACIFIC JOURNAL OF TROPICAL MEDICINE
Volume 5, Issue 8, Pages 645-650Publisher
WOLTERS KLUWER MEDKNOW PUBLICATIONS
DOI: 10.1016/S1995-7645(12)60132-1
Keywords
Nasopharyngeal carcinoma; Pim-1; Quercetagetin; Cell proliferation; Cell migration
Funding
- Doctoral Program of Guangdong Medical College [B2010013]
- National Natural Science Foundation of China [81000073]
- Natural Foundation of Hainan Province of China [811197, 310043, 811201]
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Objective: To explore the role of proto-oncogene Pim-1 in the proliferation and migration of nasopharyngeal carcinoma (NPC) cells. Methods: Pim-1 expressions in NPC cell lines CNE1, CNE1-GL, CNE-2Z and C666-1 were examined by RT-PCR, western blotting and immunoflucesence, respectively. After CNE1, CNE1-GL and C666-1 cells were treated with different concentrations of Pim-1 special inhibitor, quercetagetin, the cell viability, colony formation rate and migration ability were analyzed. Results: Pim-1 expression was negative in well-differentiated CNE1 cells, whereas expressed weakly positive in poor-differentiated CNE-2Z cells and strongly positive in undifferentiated C666-1 cells. Interestingly, CNE1-GL cells that derived from CNE1 transfected with an Epstein Barr virus latent membrane protein-1 over-expression plasmid displayed stronger expression of Pim-1. Treatment of CNE1-GL and C666-1 cells with quercetagetin significantly decreased the cell viability, colony formation rate and migration ability but not the CNE1 cells. Conclusions: These findings suggest that Pim-1 overexpression contributes to NPC proliferation and migration, and targeting Pim-1 may be a potential treatment for anti-Pim-1 expressed NPCs.
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