Journal
ARTHRITIS RESEARCH & THERAPY
Volume 15, Issue 5, Pages -Publisher
BMC
DOI: 10.1186/ar4352
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Funding
- Fondation Groupama pour la sante (Bourse espoir 2009 de la Fondation Groupama pour la sante)
- Societe Francaise de Rhumatologie and the Association des Sclerodermiques de France
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Introduction: B lymphocytes might play a pathogenic role in dermal fibrosis in systemic sclerosis (SSc). B-cell activating factor (BAFF), a key cytokine for B-cell activation, is increased in the serum and the skin of patients with SSc. However, the ability of B cells directly to stimulate dermal fibroblasts and the role of BAFF are not fully understood. We therefore investigated the involvement of B cells and BAFF in the expression of collagen and profibrotic markers by dermal fibroblasts. Methods: Cocultures of blood B cells from healthy blood donors and normal or SSc dermal fibroblasts stimulated with anti-IgM and BAFF were performed. Alpha-SMA, TIMP1, MMP9, COL1A1, COL1A2, and COL3A1 mRNA expression were determined by quantitative RT-PCR. Soluble collagen, BAFF, IL-6, IL-1 beta, TGF-beta 1, and CCL2 protein secretion were assessed. Results: Coculture of blood B cells and dermal fibroblasts isolated from SSc patients induced IL-6, TGF-beta 1, CCL2, and collagen secretion, as well as Alpha-SMA, TIMP1, and MMP9 expression in dermal fibroblasts. Transwell assays demonstrated that this induction was dependent on cell-cell contact. Addition of anti-IgM and BAFF to the coculture increased IL-6, CCL2, TGF-beta 1, and collagen secretion. B cell-and BAFF-induced collagen secretion was highly reduced by anti-TGF-beta 1 antibodies. Conclusions: Our results showed for the first time a direct role of B cells on the production of collagen by dermal fibroblasts, which is further enhanced by BAFF. Thus, these results demonstrate a new pathogenic role of B cells and BAFF in fibrosis and systemic sclerosis.
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