Journal
BIOFABRICATION
Volume 5, Issue 1, Pages -Publisher
IOP PUBLISHING LTD
DOI: 10.1088/1758-5082/5/1/015012
Keywords
-
Funding
- MEXT/JSPS KAKENHI from the Ministry of Education, Culture, Sports, Science and Technology of Japan [24360340]
Ask authors/readers for more resources
We report a method for preparing cell-laden hydrogel tubes. This method uses a coaxial double-orifice spinneret, simpler than triple-orifice spinnerets which have been used for preparing similar constructs. The intended application was to create a template for preparing filament-like structures composed of two heterogeneous living cell layers. An aqueous solution containing an alginate derivative possessing phenolic hydroxyl moieties (Alg-Ph), catalase and horseradish peroxidase (HRP) was extruded into an ambient flow of H2O2 aqueous solution. This operation enabled the Alg-Ph solution to be gellable through a HRP-catalyzed reaction, cross-linking the Ph moieties together. By altering flow rates of the Alg-Ph and H2O2 solutions along with the concentrations of catalase and H2O2, the diameter and membrane thickness of the hydrogel tubes were controllable between 250-550 mu m and 70-140 mu m, respectively. The viability of the HeLa cells enclosed in the hydrogel tubes with a diameter of 300 mu m and a membrane thickness of 80 mu m was 95.4%. Subsequently, the enclosed HeLa cells grew and filled the hollow core. A filament-like structure of HeLa cells covered with a layer of fibroblast 10T1/2 cells was obtained when confluency of fibroblast 10T1/2 cells was reached and the hydrogel matrix was degraded with alginate lyase.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available