Journal
BIOFABRICATION
Volume 4, Issue 3, Pages -Publisher
IOP PUBLISHING LTD
DOI: 10.1088/1758-5082/4/3/035002
Keywords
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Funding
- National Institutes of Health [R01HL076485, P41 EB002520]
- NSERC Strategic grant [STPGP 381002-09]
- NSERC-CIHR Collaborative Health Research grant [CHRPJ 385981-10]
- NSERC Discovery grant [RGPIN 326982-10]
- Discovery Accelerator Supplement [RGPAS 396125-10]
- Ontario Centres of Excellence International Scholarship
- Ontario Graduate Scholarship in Science and Technology (OGSST)
- Ontario Graduate Scholarship (OGS)
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We previously reported that preculture of fibroblasts (FBs) and endothelial cells (ECs) prior to cardiomyocytes (CMs) improved the structural and functional properties of engineered cardiac tissue compared to culture of CMs alone or co-culture of all three cell types. However, these approaches did not result in formation of capillary-like cords, which are precursors to vascularization in vivo. Here we hypothesized that seeding the ECs first on Matrigel and then FBs 24 h later to stabilize the endothelial network (sequential preculture) would enhance cord formation in engineered cardiac organoids. Three sequential preculture groups were tested by seeding ECs (D4T line) at 8%, 15% and 31% of the total cell number on Matrigel-coated microchannels and incubating for 24 h. Cardiac FBs were then seeded (32%, 25% and 9% of the total cell number, respectively) and incubated an additional 24 h. Finally, neonatal rat CMs (60% of the total cell number) were added and the organoids were cultivated for seven days. Within 24 h, the 8% EC group formed elongated cords which eventually developed into beating cylindrical organoids, while the 15% and 31% EC groups proliferated into flat EC monolayers with poor viability. Excitation threshold (ET) in the 8% EC group (3.4 +/- 1.2 V cm(-1)) was comparable to that of the CM group (3.3 +/- 1.4 V cm(-1)). The ET worsened with increasing EC seeding density (15% EC: 4.4 +/- 1.5 V cm(-1); 31% EC: 4.9 +/- 1.5 V cm(-1)). Thus, sequential preculture promoted vascular cord formation and enhanced architecture and function of engineered heart tissues.
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