Journal
VIRUSES-BASEL
Volume 10, Issue 8, Pages -Publisher
MDPI
DOI: 10.3390/v10080416
Keywords
RNA silencing; plant protection; resistance; plant viruses; viral suppressors
Categories
Funding
- National Funds through FCT-Foundation for Science and Technology [UID/AGR/00115/2013]
- European Union through the European Regional Development Fund under the ALENTEJO 2020 (Regional Operational Program of the Alentejo) through the project Enhancing the Performance of Portuguese Olive Cultivars (OLEAVALOR) [ALT20-03-0145-FEDER-000014]
- project Control of olive anthracnose through gene silencing and gene expression using a plant virus vector [ALT20-03-0145-FEDER-028263]
- project Development of a new virus-based vector to control TSWV in tomato plants [ALT20-03-0145-FEDER-028266]
- European Union through the European Regional Development Fund under the ALENTEJO 2020 (Regional Operational Program of the Alentejo), ALGARVE 2020 (Regional Operational Program of the Algarve)
- European Union through the Foundation for Science and Technology
- Foundation for Science and Technology (FCT) [SFRH/BPD/76194/2011]
- QREN-POPH-Typology 4.1-MES
- European Social Fund
- Fundação para a Ciência e a Tecnologia [UID/AGR/00115/2013] Funding Source: FCT
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RNA silencing is an important defense mechanism in plants, yet several plant viruses encode proteins that suppress this mechanism. In this study, the genome of the Olive mild mosaic virus (OMMV) was screened for silencing suppressors. The full OMMV cDNA and 5 OMMV open reading frames (ORFs) were cloned into the Gateway binary vector pK7WG2, transformed into Agrobacterium tumefaciens, and agroinfiltrated into N. benthamiana 16C plants. CP and p6 showed suppressor activity, with CP showing significantly higher activity than p6, yet activity that was lower than the full OMMV, suggesting a complementary action of CP and p6. These viral suppressors were then used to induce OMMV resistance in plants based on RNA silencing. Two hairpin constructs targeting each suppressor were agroinfiltrated in N. benthamiana plants, which were then inoculated with OMMV RNA. When silencing of both suppressors was achieved, a significant reduction in viral accumulation and symptom attenuation was observed as compared to those of the controls, as well as to when each construct was used alone, proving them to be effective against OMMV infection. This is the first time that a silencing suppressor was found in a necrovirus, and that two independent proteins act as silencing suppressors in a virus member of the Tombusviridae family.
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