Journal
VIRUSES-BASEL
Volume 6, Issue 1, Pages 340-353Publisher
MDPI
DOI: 10.3390/v6010340
Keywords
Puumala virus; Poland; bank vole; Clethrionomys (Myodes) glareolus
Categories
Funding
- FRISC project (Polish Ministry of Science)
- FRISC project (EEA/Norwegian Financial Mechanism)
- Jagiellonian University [DS-758757]
- German Academic Exchange Service (DAAD), Eastern and southern Africa [413, A/09/90015]
Ask authors/readers for more resources
Puumala virus (PUUV) causes mild to moderate cases of haemorrhagic fever with renal syndrome (HFRS), and is responsible for the majority of hantavirus infections of humans in Fennoscandia, Central and Western Europe. Although there are relatively many PUUV sequences available from different European countries, little is known about the presence of this virus in Poland. During population studies in 2009 a total of 45 bank voles were trapped at three sites in north-eastern Poland, namely islands on Dejguny and Dobskie Lakes and in a forest near Mikolajki. S and M segment-specific RT-PCR assays detected PUUV RNA in three animals from the Mikolajki site. The obtained partial S and M segment sequences demonstrated the highest similarity to the corresponding segments of a PUUV strain from Latvia. Analysis of chest cavity fluid samples by IgG ELISA using a yeast-expressed PUUV nucleocapsid protein resulted in the detection of two seropositive samples, both being also RT-PCR positive. Interestingly, at the trapping site in Mikolajki PUUV-positive bank voles belong to the Carpathian and Eastern genetic lineages within this species. In conclusion, we herein present the first molecular evidence for PUUV in the rodent reservoir from Poland.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available