Journal
FOOD AND ENVIRONMENTAL VIROLOGY
Volume 5, Issue 3, Pages 180-183Publisher
SPRINGER
DOI: 10.1007/s12560-013-9116-2
Keywords
Mengovirus; Real-time RT-PCR; Sludge; Morocco
Funding
- Medical Virology & BSL3 Laboratory, Institut Pasteur du Maroc, Casablanca, Morocco
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In the virus detection protocol for sludge, the viral elution step from solids to solution is critical. In this study, mengoviruses were detected in artificially contaminated sludge with a qRT-PCR assay. The viral yields ranged between 19 and 66 % for 60 % sludge. This study demonstrates that mengovirus can be used as a sample process control for analysis of sewage sludge.
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