Journal
FOOD AND ENVIRONMENTAL VIROLOGY
Volume 1, Issue 2, Pages 57-65Publisher
SPRINGER
DOI: 10.1007/s12560-009-9008-7
Keywords
F+ RNA bacteriophages; Virus surrogate; Real-time RT-PCR; Feline calicivirus; Sample process control
Funding
- Agriculture and Agri-Food Canada Research Branch [75, 162]
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There are increasing concerns of zoonotic transmission of some animal enteric viruses, such as calicivirus, hepatitis E virus, and rotavirus, which are closely related to human pathogenic strains. Most enteric viruses are detected by molecular techniques because they cannot be cultured. Surrogates such as F-RNA coliphages are cultivable but few molecular methods exist. Individual real-time TaqMan RT-PCR assays for the replicase gene of F-RNA coliphage genogroups I and IV were developed and multiplexed with a real-time TaqMan RT-PCR assay for feline calicivirus as a sample process control for the simultaneous detection and enumeration of genogroup I and IV F-RNA coliphages. Genogroup IV were successfully detected with the multiplexed assay in 80% of fecal samples that contained F-RNA coliphage levels >= 3.2 log plaque forming units (pfu). F-RNA coliphage were at or below the limit of detection in most fecal samples when levels were <= 4 log pfu/g.
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