Journal
ZEITSCHRIFT FUR NATURFORSCHUNG SECTION B-A JOURNAL OF CHEMICAL SCIENCES
Volume 64, Issue 3, Pages 331-334Publisher
WALTER DE GRUYTER GMBH
DOI: 10.1515/znb-2009-0314
Keywords
Phospholipids; MALDI-TOF MS; Adducts; Matrix Clusters; Positive Ion Mode; Negative Ion Mode
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Funding
- German Research Council [Schi 476/5-1]
- Federal Ministry of Education and Research [0313836]
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It is usually accepted that neutral phospholipids (PLs) generate singly positively charged ions, whereas negative PLs are easily detectable in the negative ion mode when analysed by matrix-assisted laser desorption and ionisation time-of-flight mass spectrometry (MALDI-TOF MS). In this study, we demonstrate that some caution is required in the interpretation of MALDI-TOF mass spectra of PLs, since also neutral PLs have appeared to be detectable in the negative ion mode as well. Neutral and negatively charged phospholipids can generate adducts with the most commonly used matrix - 2,5-dihydroxybenzoic acid - yielding singly negatively charged ions that are detectable in the spectra. This further contributes to the complexity of the spectra and potentially leads to severe misinterpretation, particularly when unknown mixtures of PLs are analysed by MALDI-TOF MS.
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