Journal
ZEBRAFISH
Volume 7, Issue 2, Pages 199-204Publisher
MARY ANN LIEBERT INC
DOI: 10.1089/zeb.2009.0632
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Funding
- Association pour la Recherche sur le Cancer (ARC) [3787]
- ANR [PCV 2008]
- NABI CNRS-Weizmann Institute Program
- Ministere de la Recherche
- National Science Foundation [PHY05-51164]
- PUF ENS-UCLA
- Volkswagen Association
- EU [LSHC-CT-2003-503466]
- Center for Protein Science-Munich
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We implemented a noninvasive optical method for the fast control of Cre recombinase in single cells of a live zebrafish embryo. Optical uncaging of the caged precursor of a nonendogeneous steroid by one-or two-photon illumination was used to restore Cre activity of the CreER(T2) fusion protein in specific target cells. This method labels single cells irreversibly by inducing recombination in an appropriate reporter transgenic animal and thereby can achieve high spatiotemporal resolution in the control of gene expression. This technique could be used more generally to investigate important physiological processes (e.g., in embryogenesis, organ regeneration, or carcinogenesis) with high spatiotemporal resolution (single cell and 10-min scales).
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