Journal
ZEBRAFISH
Volume 6, Issue 1, Pages 69-77Publisher
MARY ANN LIEBERT, INC
DOI: 10.1089/zeb.2008.0555
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Funding
- NATIONAL EYE INSTITUTE [T32EY007133] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM063904, R56GM063904] Funding Source: NIH RePORTER
- NEI NIH HHS [T32 EY007133-17, T32 EY007133] Funding Source: Medline
- NIGMS NIH HHS [R56 GM063904-08, R56 GM063904, R01 GM063904] Funding Source: Medline
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Morpholino oligonucleotides are the most common anti-sense knockdown'' technique used in zebrafish (Danio rerio). This review discusses common practices for the design, preparation, and deployment of morpholinos in this vertebrate model system. Off-targeting effects of morpholinos are discussed as well as method to minimize this potentially confounding variable via co-injection of a tP53-targeting morpholino. Finally, new uses of morpholinos are summarized and contextualized with respect to the complementary, DNA-based knockout technologies recently developed for zebrafish.
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