Journal
YEAST
Volume 28, Issue 12, Pages 809-814Publisher
WILEY
DOI: 10.1002/yea.1907
Keywords
ammonium sulphate; real-time PCR; FLO11; RAS2
Categories
Funding
- Legge Regionale Promozione della ricerca scientifica e dell'innovazione tecnologica in Sardegna
- O.I.V. (Organisation internationale de la vigne et vin)
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Airliquid biofilm formation appears to be an adaptive mechanism that promotes foraging of Saccharomyces cerevisiae flor strains in response to nutrient starvation. The FLO11 gene plays a central role in this phenotype as its expression allows yeast cells to rise to the liquid surface. Here, we investigated the role of ammonium depletion in airliquid biofilm formation and FLO11 expression in a S. cerevisiae flor strain. The data obtained show that increasing ammonium concentrations from 0 to 450?m m reduce airliquid biofilm in terms of biomass and velum formation and correlate with a reduction of FLO11 expression. Rapamycin inhibition of the TOR pathway and deletion of RAS2 gene significantly reduced biofilm formation and FLO11 expression. Taken together, these data suggest that ammonium depletion is a key factor in the induction of airliquid biofilm formation and FLO11 expression in S. cerevisiae flor strains. Copyright (c) 2011 John Wiley & Sons, Ltd.
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