Journal
YEAST
Volume 28, Issue 11, Pages 795-798Publisher
WILEY-BLACKWELL
DOI: 10.1002/yea.1905
Keywords
LiAc; yeast; Saccharomyces cerevisiae; protein extraction
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Funding
- National Natural Science Foundation of China [30870119]
- National High Technology Research and Development Program of China (863 Program) [2009AA10Z110]
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A new method for protein extraction from yeast Saccharomyces cerevisiae cells is described. This method involves the use of LiAc and NaOH to enhance the permeability of yeast cell wall prior to protein extraction with SDS-PAGE sample buffer. It was safe and efficient compared to other methods reported so far in the literature. The proteins extracted with this new method retained their immunoreactive properties and are suitable for most applications in molecular biology studies. Copyright (C) 2011 John Wiley & Sons, Ltd.
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