Journal
YEAST
Volume 26, Issue 1, Pages 67-73Publisher
WILEY-BLACKWELL
DOI: 10.1002/yea.1652
Keywords
Schizosaccharomyces; glutathione; uptake; oligopeptides
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A protein involved in the transport of glutathione has been identified, cloned and characterized from the fission yeast Schizosaccharomyces pombe. Database searches revealed the Sz. pombe ORF SPAC29B12.10c as a close homologue to several members of the OPT family, including the Saccharomyces cerevisiae high-affinity glutathione transporter Hgt1p. The gene product of SPAC29B12.10c has been identified as a protein, named SpOPT1, localized within the plasma membrane, transporting the tripeptide glutathione. Disruption of SPAC29B12.10c led to strains inable to grow on media containing glutathione as a sole source of sulphur, due to the inability to internalize the tripeptide. Disruptants contained significantly less glutathione than wild-type cells. Furthermore, Delta Spopt1 strains were non-viable in a glutathione biosynthesis-defective (Delta gsh2) background. However, it was possible to complement the disruption of Spopt1 by overexpressing the intact ORF in the disrupted strain. Copyright (C) 2009 John Wiley & Sons, Ltd.
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