Journal
YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN
Volume 129, Issue 3, Pages 289-295Publisher
PHARMACEUTICAL SOC JAPAN
DOI: 10.1248/yakushi.129.289
Keywords
green fluorescent protein; luciferase; mRNA; mitochondria
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Funding
- Japan Science and Technology Agency (JST)
- Japan Society for the Promotion of Science (JSPS)
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One of the most challenging researches in current biology and medicinal chemistry is to understand how individual cellular molecules interact together in living cells. To visualize such molecules, genetically-encoded reporters have been used widely. The most common reporters are firefly luciferase, renilla luciferase, green fluorescent protein (GFP) and its variants with various spectral properties. In this review, novel design of split GFP and split luciferase is described. The principle is based on reconstitution of the split-reporter fragments when they are brought together into close proximity. The reconstitution methods are used for screening organ elle-localized proteins, imaging dynamics of nuclear proteins and mRNAs in living cells, and visualizing protease activities in living animals. These methods are generally applicable for imaging of complex cellular processes and evaluating chemical effects in living cells and animals.
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