Journal
WORLD JOURNAL OF SURGICAL ONCOLOGY
Volume 12, Issue -, Pages -Publisher
BIOMED CENTRAL LTD
DOI: 10.1186/1477-7819-12-258
Keywords
PPM1D; lung cancer; shRNA; cell proliferation; cell cycle
Funding
- National Natural Science Foundation of China [81202550, 81350110521]
- China Postdoctoral Science Foundation [2013 M541548]
- Tongji University [1500219079]
- National Science Foundation 973 program [2010CB945600, 2011CB965100]
- Shanghai Municipal Commission of Health and Family Planning [2012J011A]
- Science and Technology Commission Of Shanghai Municipality [12ZR1423400]
Ask authors/readers for more resources
Background: PPM1D (protein phosphatase, Mg2+/Mn2+ dependent, 1D) has been reported to be involved in multiple human tumors. This study was designed to investigate the functional role of PPM1D in lung cancer cells. Methods: Expression levels of PPM1D were analyzed in A549 and H1299 cells by real-time PCR and Western blotting. Lentivirus-mediated short hairpin RNA (shRNA) was used to knock down PPM1D expression in both cell lines. The effects of PPM1D on lung cancer cell growth were investigated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), colony formation and flow cytometry assays. Results: Knockdown of PPM1D in lung cancer cells resulted in decreased cell proliferation and impaired colony formation ability. Moreover, flow cytometry analysis showed that knockdown of PPM1D arrested cell cycle at the G(0)/G(1) phase. Furthermore, PPM1D silencing downregulated the expression of cyclin B1 in H1299 cells. Therefore, it is reasonable to speculate that the mechanisms by which PPM1D knockdown alleviates cell growth may be partly via the induction of cell cycle arrest due to the suppression of cyclin B1. Conclusions: These results suggest that PPM1D silencing by RNA interference (RNAi) may be a potential therapeutic approach for the treatment of lung cancer.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available