Journal
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
Volume 31, Issue 2, Pages 371-377Publisher
SPRINGER
DOI: 10.1007/s11274-014-1789-7
Keywords
Biotransformation; Cytochrome P-450cam; Hexachlorobenzene; Pentachlorophenol; Sphingobium chlorophenolicum ATCC 39723
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Funding
- Natural Science Foundation of Hubei Province of China [2008CDB067]
- National Natural Science Foundation of China (NSFC) [31270112]
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A consortium comprised of an engineered Escherichia coli DH5 alpha and a natural pentachlorophenol (PCP) degrader, Sphingobium chlorophenolicum ATCC 39723, was assembled for degradation of hexachlorobenzene (HCB), a persistent organic pollutant. The engineered E. coli strain, harbouring a gene cassette (camA (+) camB (+) camC) that encodes the F87W/Y96F/L244A/V247L mutant of cytochrome P-450cam (CYP101), oxidised HCB to PCP. The resulting PCP was then further completely degraded by ATCC 39723. The results showed that almost 40 % of 4 mu M HCB was degraded by the consortium at a rate of 0.033 nmol/mg (dry weight)/h over 24 h, accompanied by transient accumulation and immediate consumption of the intermediate PCP, detected by gas chromatography. In contrast, in the consortium comprised of Pseudomonas putida PaW340 harbouring camA (+) camB (+) camC and ATCC 39723, PCP accumulated in PaW340 cells but could not be further degraded, which may be due to a permeability barrier of Pseudomonas PaW340 for PCP transportation. The strategy of bacterial co-culture may provide an alternative approach for the bioremediation of HCB contamination.
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