Journal
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
Volume 30, Issue 1, Pages 201-211Publisher
SPRINGER
DOI: 10.1007/s11274-013-1440-z
Keywords
Laccase; Heterologous expression; Phanerochaete flavido-alba; Solvent-tolerant; Dye biotransformation
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Funding
- Spanish Ministry of Economy and Competitiveness [AGL2006-11697]
- Spanish Ministry of Education and Science Ayudas para la movilidad de estudiantes para la obtencion de la mencion europea en el titulo de doctor''
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We investigated the expression of Phanerochaete flavido-alba laccase gene in Aspergillus niger and the physical and biochemical properties of the recombinant enzyme (rLac-LPFA) in order to test it for synthetic dye biotransformation. A. niger was able to produce high levels of active recombinant enzyme (30 mgL(-1)), whose identity was further confirmed by immunodetection using Western blot analysis and N-terminal sequencing. Interestingly, rLac-LPFA exhibited an improved stability at pH (2-9) and organic solvents tested. Furthermore, the percentage of decoloration and biotransformation of synthetic textile dyes, Remazol Brilliant Blue R (RBBR) and Acid Red 299 (NY1), was higher than for the native enzyme. Its high production, simple purification, high activity, stability and ability to transform textile dyes make rLac-LPFA a good candidate for industrial applications.
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