Journal
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
Volume 28, Issue 6, Pages 2411-2416Publisher
SPRINGER
DOI: 10.1007/s11274-012-1050-1
Keywords
Agaricus brasiliensis; A. subrufescens; Royal sun agaricus; Almond Portobello; Preservation methods; Mycelial vitality
Categories
Funding
- Fundacao de Amparo a Pesquisa de Minas Gerais-FAPEMIG (Research Support Foundation of Minas Gerais)
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior-CAPES (Coordination of Improvement of Higher Education Personnel)
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cultures quickly lose viability when stored at cool temperatures, even for a short period of time. We evaluated several low-cost preservation methods using varied substrates, preservation solutions, and storage temperatures. was intolerant to freezing temperatures, making liquid nitrogen use and deep-freezing methods impossible for its preservation. The best preservation conditions for the CS1 strain tested in this study were obtained by using rice as substrate and water as preservation solution, with storage at room temperature or when using soil, mushroom cultivation compost, or rice and stored at 10 A degrees C without preservation solution. Those cultures that were reactivated showed the same productivity attributes as the control. In addition, no effect on productivity or biological efficiency was observed through successive subculturing of the strain (CS1). Parboiled rice was successfully used for other strains (CS2, CS5, CS7, CS9, and CS10), and also for , -, and .
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