Journal
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
Volume 26, Issue 6, Pages 1135-1141Publisher
SPRINGER
DOI: 10.1007/s11274-009-0281-2
Keywords
Deep-sea bacterium; Halomonas sp.; V3a '; Bioflocculant; Flocculating characteristics; Flocculating mechanism
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Funding
- Ocean Project of China [DYXM-115-02-02-05]
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A novel bioflocculant HBF-3 produced by deep-sea bacterium mutant Halomonas sp. V3a' was investigated with regard to its flocculating characteristics and mechanism. 4.0 m g l(-1) HBF-3 showed the maximum flocculating activity of 96.9% in 5.0 g l(-1) Kaolin suspension containing 11.25 mM CaCl2, and that its flocculating activity was more than 90% within 5-40A degrees C and over 80% in a wide pH range (3.0-11.0). Chemical analyses indicated that the biopolymer HBF-3 was mainly a polysaccharide, including neutral sugar residues (20.6%), uronic acid residues (7.6%), amino sugar residues (1.6%) and sulfate groups (5.3%). Fourier transform infrared (FTIR) spectrum showed the presence of carboxyl and hydroxyl groups in HBF-3 molecular. The average molecular mass of HBF-3, as determined by gel filtration chromatography (GFC), was approximately 590 kDa. Flocculation of Kaolin suspension with HBF-3 acted as a model to explore the flocculating mechanism in which bridging mediated by Ca2+ was proposed as the primary action based upon the experimental observations.
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