4.5 Article

A marine bacterium accumulates polyhydroxyalkanoate consisting of mainly 3-hydroxydodecanoate and 3-hydroxydecanoate

Journal

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
Volume 26, Issue 6, Pages 1149-1153

Publisher

SPRINGER
DOI: 10.1007/s11274-009-0282-1

Keywords

Polyhydroxyalkanoate; Psychrotrophic; Exopolysaccharide; Pseudoaltermonas

Funding

  1. National HighTech Research and Development Plan of China [2006AA02Z218]
  2. National Basic Research Program of China [2007CB707803]
  3. National Natural Science Foundation of China [30870022]

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A deep-sea psychrotrophic bacterium Pseudoaltermonas sp. SM9913, isolated from abyssalbenthic sediments in Bohai Sea, was found to be able to synthesize polyhydroxyalkanoate (PHA) polymer consisting of mainly 3-hydroxydecanoate and 3-hydroxydodecanoate. PHA accumulation in Pseudoaltermonas sp. SM9913 was confirmed by transmission electron microscope, Nile red dye and gas chromatography/mass spectral. The PHA content was determined as high as 2-3% of cell dry weight when the strain was grown in a sea water-based liquid medium. The relationship between PHA and exopolysaccharide accumulation in this strain was discussed.

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