Journal
WORLD JOURNAL OF GASTROENTEROLOGY
Volume 20, Issue 32, Pages 11347-11355Publisher
BAISHIDENG PUBLISHING GROUP INC
DOI: 10.3748/wjg.v20.i32.11347
Keywords
Advanced gastric cancer; Cytoreductive surgery; Hyperthermic intraperitoneal chemotherapy miR-218; Multi-drug resistance; MicroRNA
Categories
Funding
- Start-up Funds of Guangzhou Medical College, Guangdong Province, China [2012C66, 2012C69]
- Guangdong Province Natural Science Fund [S2013010016662]
- National Natural Science Foundation of China [81201932, 81372493]
Ask authors/readers for more resources
AIM: To investigate the molecular mechanisms of miRNA in advanced gastric cancers (AGCs) before and after cytoreductive surgery (CRS) + hyperthermic intraperitoneal chemotherapy (HIPEC). METHODS: A miRNA microarray containing human mature and precursor miRNA sequences was used to compare expression profiles in serum samples of 5 patients with AGC before and after CRS + HIPEC. The upregulation of miR-218 was confirmed by real-time reverse transcription polymerase chain reaction and its expression was analyzed in SGC7901 gastric cancer cells. RESULTS: miRNA microarray chip analysis found that the level of miR-218 expression was upregulated more than 8 fold after CRS + HIPEC. Furthermore, miR-218 increased gastric cancer cell chemosensitivity to cisplatin in vitro and inhibited gastric cell tumor growth in nude mice in vivo (0.5 vs 0.78, P < 0.05). CONCLUSION: Our results indicated that targeting miR-218 may provide a strategy for blocking the development of gastric cancer and reverse the multi-drug resistance of gastric cell lines. (C) 2014 Baishideng Publishing Group Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available