Journal
WORLD JOURNAL OF GASTROENTEROLOGY
Volume 18, Issue 18, Pages 2180-2187Publisher
BAISHIDENG PUBL GRP CO LTD
DOI: 10.3748/wjg.v18.i18.2180
Keywords
Inflammation; Microbiota; Tight junction protein; Side-stream smoking; Intestine
Categories
Funding
- INBRE [P20RR016474]
- USDA-NRI [2008-35203-19084]
- USDA-AFRI [2009-65203-05716]
Ask authors/readers for more resources
AIM: To investigate the effect of side-stream smoking on gut microflora composition, intestinal inflammation and expression of tight junction proteins. METHODS: C57BL/6 mice were exposed to side-stream cigarette smoking for one hour daily over eight weeks. Cecal contents were collected for microbial composition analysis. Large intestine was collected for immunoblotting and quantitative reverse transcriptase polymerase chain reaction analyses of the inflammatory pathway and tight junction proteins. RESULTS: Side-stream smoking induced significant changes in the gut microbiota with increased mouse intestinal bacteria, Clostridium but decreased Fermicutes (Lactoccoci and Ruminococcus), Enterobacteriaceae family and Segmented filamentous baceteria compared to the control mice. Meanwhile, side-stream smoking inhibited the nuclear factor-kappa B pathway with reduced phosphorylation of p65 and I kappa B alpha, accompanied with unchanged mRNA expression of tumor necrosis factor-alpha or interleukin-6. The contents of tight junction proteins, claudin3 and ZO2 were up-regulated in the large intestine of mice exposed side-stream smoking. In addition, side-stream smoking increased c-Jun N-terminal kinase and p38 MAPK kinase signaling, while inhibiting AMP-activated protein kinase in the large intestine. CONCLUSION: Side-stream smoking altered gut microflora composition and reduced the inflammatory response, which was associated with increased expression of tight junction proteins. (C) 2012 Baishideng. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available