Journal
WORLD JOURNAL OF GASTROENTEROLOGY
Volume 17, Issue 1, Pages 79-88Publisher
BAISHIDENG PUBLISHING GROUP INC
DOI: 10.3748/wjg.v17.i1.79
Keywords
Esophageal squamous cell carcinoma; MicroRNA; miR-143; miR-145; Tumor invasion depth
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Funding
- NSFC [U0932001]
- National Natural Science Foundation of China [30900560]
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AIM: To investigate the expression profile of miRNA in esophageal squamous cell carcinoma (ESCC). METHODS: The expression profile of miRNA in ESCC tissues was analyzed by miRNA microarray. The expression levels of miR-143 and miR-145 in 86 ESCC patients were determined by real-time polymerase chain reaction (PCR) using TaqMan assay. The mobility effect was estimated by wound-healing using esophageal carcinoma cells transfected with miRNA expression plasmids. RESULTS: A set of miRNAs was found to be deregulated in the ESCC tissues, and the expression levels of miR-143 and -145 were significantly decreased in most of the ESCC tissues examined. Both miR-143 and nniR-145 expression correlated with tumor invasion depth. The transfection of human esophageal carcinoma cells with miR-143 and nniR-145 expression plasmids resulted in a greater inhibition of cell mobility, however, the protein level of the previously reported target of miR-145, FSCN1, did not show any significant downregulation. CONCLUSION: These findings suggest that the deregulation of miRNAs plays an important role in the progression of ESCC. Both miR-143 and miR-145 might act as anti-oncomirs common to ESCC. (c) 2011 Baishideng. All rights reserved.
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